The Effect of Invigoration Using Polyethylene Glycol And Ultra Fine Bubble on Improving of Sengon Seeds (Falcataria Moluccana Miq.) Quality After Two Years Storage
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Abstract
Utilization of seeds storage results in decreased plant productivity. The increase can be used by treating seeds before planting through the technique of invigoration. This purpose of the study is to assess the effectiveness of invigoration methods using polyethylene glycol (PEG 6000) and ultrafine bubbles (UFB) to improve viability and vigor of sengon seeds (Falcataria moluccana). Seed agieng using 96 % ethanol was carried out to obtain the diversity of seedlot viability as the materials for testing the effectiveness of invigoration treatments. A complete random design was used to test the effectiveness of 5 invigoration treatments, i.e., seed without invigoration treatment, soaking in PEG 6000 -0.8 MPa, soaking in PEG 6000 -1.2 Mpa, soaking in UFB water injected by environmental air, and soaking in UFB water injected by oxygen 99 %, with soaking time is 24 hours for each treatment. Seed agieng resulted three classes of seed viability, i.e. 62 % (initial seed), 83 % (seed agieng for 30 minutes) and 57 % (seed agieng for 60 minutes). In the condition of seed germination before treatment (DBA) 57 % and DBA 62 %, invigoration treatments were significantly affected on seed germination capacity, but not significantly different in DBA 83 %. The soaking treatment of UFB injected by oxigen 99 % was able to improve the germination parameters (germination capacity, germination rate, and vigor index) on the sengon seeds with DBA 57 %. For seeds with DBA 62 %, the soaking treatment in UFB injected by environmental air was provided the best germination capacity, T50, and vigor index. The treatment of UFB injected by oxygen 99 % was more effective to improve the seed with very low viability and vigor (DBA 57 %). In general, improving of seed viability and vigor is more effective by using UFB than PEG 6000.
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Agurahe, L., Rampe, H. L., & Mantir, F. R. (2019). Pematahan dormansi benih pala (Myristica fragrans Houtt.) menggunakan hormon giberealin. Jurnal Ilmiah Farmasi, 8(1), 30–40.
Ahmed, A. K. A., Shi, X., Hua, L., Manzueta, L., Qing, W., Marhaba, T., & Zhang, W. (2018). Influences of air, oxygen, nitrogen, and carbon dioxide nanobubbles on seed germination and plant growth. Journal of Agricultural and Food Chemistry, 66(20), 5117–5124. https://doi.org/10.1021/acs.jafc.8b00333
Balestrazzi, A., Confalonieri, M., Macovei, A., & Carbonera, D. (2011). Seed imbibition in medicago truncatula gaertn.: Expression profiles of DNA repair genes in relation to PEG-mediated stress. Journal of Plant Physiology, 168(7), 706–713. https://doi.org/10.1016/j.jplph.2010.10.008
Baskorowati, L., Setiadi, D., & Fauzi, M. A. (2017). Viabilitas biji sengon penyimpanan 6 bulan dan 23 tahun. dalam Nurcahyanto, G. & Kartikasari, S. (eds), Seminar Nasional Pendidikan Biologi dan Saintek II, Surakarta (p.62–69), Surakarta: Universitas Muhammadiyah Surakarta.
Belo, M., & Suwarno, F. C. (2012). Penurunan viabilitas benih padi (Oryza sativa L.) melalui beberapa metode pengusangan cepat. Agronomi Indonesia, 40(1), 29–35.
Diniyati, D., Achmad, B., & Santoso, H. B. (2013). Analisis finansial agroforestry sengon di Kabupaten Ciamis (Study kasus di Desa Ciomas Kecamatan Panjalu). Jurnal Penelitian Agroforestry, 1(1), 13–30.
Farooq, M., Basra, S. M. A., Rehman, H., & Mehmood, T. (2006). Germination and early seedling growth as affected by pre-sowing ethanol seed treatments in fine rice. International Journal of Agriculture and Biology, 8(1), 19–22.
Fata, N. A. N., Supriyanto, Rustam, E., & Sudrajat, D. J. (2020). Invigorasi benih jabon putih (Neolamarckia cadamba (Roxb.) Bosser) menggunakan polyethylene glycol dan ultra fine bubbles. Jurnal Perbenihan Tanaman Hutan, 8(1), 11–24.
Fathia, A. A., Hilwan, I., & Wibowo, C. (2019). Rehabilitasi lahan pada areal bekas terbakar dengan jenis tanah yang berbeda di Kabupaten Gunung Mas Kalimantan Tengah. Media Konservasi, 24(1), 20–28.
Gairola, K. C., Nautiyal, A. R., Sharma, G., & Dwivedi, A. K. (2011). Variability in seed characteristics of Jatropha curcas Linn from hill region of Uttarakhand. Bulletin of Environment, Pharmacology & Life Sciences, 1(1), 64–69.
Handayani, M. A. H., Pramono, E., & Hadi, M. S. (2014). Pengaruh konsentrasi etanol dan lama deraan pada viabilitas benih buncis (Phaseolus vulgaris L.). Jurnal Agrotek Tropika, 2(1), 83–88.
Ishibashi, Y., Tawaratsumida, T., Zheng, S. H., Yuasa, T., & Iwaya-Inoue, M. (2009). NADPH oxidases act as key enzyme on germination and seedling growth in barley (Hordeum vulgare L.). Plant Production Science, 13(1), 45–52. https://doi.org/10.1626/pps.13.45
Khairani, Z., Syamsuddin, & Ichsan, C. N. (2016). Penggunaan polyethilen glycol (PEG 6000) untuk mengetahui vigor kekuatan tumbuh benih kedelai hitam (Glycine max (L.) Merrill) pada kondisi kekeringan. Jurnal Ilmiah Mahasiswa Pertanian Unsyiah, 1(1), 280–288.
Krisnawati, H., Varis, E., Kallio, M. H., & Kanninen, M. (2011). Paraserianthes falcataria (L.) Nielsen: Ekologi, silvikultur dan produktivitas. Bogor: CIFOR.
Liu, S., Kawagoe, Y., Makino, Y., & Oshita, S. (2013). Effects of nanobubbles on the physicochemical properties of water : The basis for peculiar properties of water containing nanobubbles. Chemical Engineering Science, 93, 250–256. https://doi.org/10.1016/j.ces.2013.02.004
Liu, S., Oshita, S., Makino, Y., Wang, Q., Kawagoe, Y., & Uchida, T. (2016). Oxidative capacity of nanobubbles and its effect on seed germination. ACS Sustainable Chemistry and Engineering, 4(3), 1347–1353. https://doi.org/10.1021/acssuschemeng.5b01368
McGiffen, M. E., & Manthey, J. A. (1996). The role of methanol in promoting plant growth: A current evaluation. Hort Science, 31(7), 1092–1096.
Michel, B. E., & Kaufmann, M. R. (1973). The osmotic potential of Polyethylene Glycol 6000. Plant Physiology, 51, 914–916.
Moritz, M., & Geszke-Moritz, M. (2013). The newest achievements in synthesis, immobilization and practical applications of antibacterial nanoparticles. Chemical Engineering Journal, 228(2013), 596–613. https://doi.org/10.1016/j.cej.2013.05.046
Nurmauli, & Nurmiaty, Y. (2010). Studi metode invigorasi pada viabilitas dua lot kedelai yang telah disimpan selama sembilan bulan. Jurnal Ilmu Pertanian Indonesia, 15(1), 20–24.
Pramono, A. A., Sudrajat, D. J., Nurhasybi, & Danu. (2016). Prinsip-prinsip cerdas Usaha Pembibitan Tanaman Hutan. Bogor: Penebar Swadaya.
Pratiwi, Narendra, B. H., Hartoyo, G. M. E., & Kalima, T. (2014). Atlas jenis-jenis pohon andalan setempat untuk rehabilitasi hutan dan lahan di Indonesia. Bogor: FORDA Press Putra.
Pujiastuti, E., & Sudrajat, D. J. (2017). Uji vigor untuk menduga perkecambahan benih dan munculnya semai normal Acacia mangium di persemaian. Jurnal Perbenihan Tanaman Hutan, 5(2), 81–94.
Putra, D. S. A., Lestari, D. A. H., & Affandi, M. I. (2015). Kelayakan finansial dan prospek pengembangan agribisnis sengon (Albizia falcataria) rakyat di Kecataman Kemiling Kota Bandar Lampung. Jurnal Ilmu-Ilmu Agribisnis, 3(4), 419–425.
Rouhi, H. R., Afshari, R. T., Moosavi, S. A., & Gharineh, M. H. (2010). Effects of osmopriming on germination and vigour traits of bersim clover (Trifolium alexandrinum L.). Notulae Scientia Biologicae, 2(4), 59–63. https://doi.org/10.15835/nsb244735
Shao, S., Meyer, C. J., Ma, F., Peterson, C. A., & Bernards, M. A. (2007). The outermost cuticle of soybean seeds: Chemical composition and function during imbibition. Journal of Experimental Botany, 58(5), 1071–1082. https://doi.org/10.1093/jxb/erl268
Siregar, I. Z., Muharam, K. F., Purwanto, Y. A., & Sudrajat, D. J. (2020). Seed germination characteristics in different storage time of gmelina arborea treated with ultrafine bubbles priming. Biodiversitas, 21(10), 4558–4564.
https://doi.org/10.13057/biodiv/d211013
Sritontip, C., Dechthummarong, C., Thonglek, V., Khaosumain, Y., & Sritontip, P. (2019). Stimulation of seed germination and physiological development in plants by high voltage plasma and fine bubbles. International Journal of Plasma Environmental Science and Technology, 12(2), 74–78.
Sudrajat, D. J., Nurhasybi, & Bramasto, Y. (2017). Standar Pengujian dan Mutu Benih Tanaman Hutan. Bogor: IPB Press.
Sudrajat, D. J, Nurhasybi, & Bramasto, Y. (2015). Teknologi penanganan benih dan bibit untuk memenuhi standar benih dan bibit bersertifikat. dalam Mindawati, N., Bramasto, Y., Pramono, A. A., Rahmat, M., (eds), Seminar Hasil Penelitian Balai Penelitian Teknologi Perbenihan Tanaman Hutan Balai Penelitian Kehutanan Palembang (p.15-26) Lampung: Pusat Penelitian dan Pengembangan Hutan.
Sudrajat, D. J, & Bramasto, Y. (2018). Perkecambahan benih tisuk (Hibiscus macrophylus Roxb.) pada beberapa perlakuan periode pencahayaan, perlakuan pendahuluan dan penyimpanan. Jurnal Perbenihan Tanaman Hutan, 6(1), 49–60. https://doi.org/10.20886/bptpth.2018.6.1.49-60
Syafrudin, & Miranda, T. (2015). Vigor benih beberapa varietas jagung pada media tanam tercemar hidrokarbon. Jurnal Floratek, 10(1), 18–25. https://doi.org/10.24815/floratek.v10i1.2326
Tomizawa, S., Imai, H., Tsukada, S., Simizu, T., Honda, F., Nakamura, M., Nagano, T., Urano, Y., Matsuoka, Y., Fukasaku, N., & Saito, N. (2005). The detection and quantification of highly reactive oxygen species using the novel HPF fluorescence probe in a rat model of focal cerebral ischemia. Neuroscience Research, 53(3), 304–313
https://doi.org/10.1016/j.neures.2005.08.002
Wahyuni, S. (2011). Peningkatan daya berkecambah dan vigor benih padi hibrida melalui invigorasi. Penelitian Pertanian Tanaman Pangan, 30(2), 83–87.
Yuanasari, B. S., Kendarini, N., & Saptadi, D. (2015). Peningkatan viabilitas benih kedelai hitam (Glycine max L. Merr) melalui invigorasi ormocontitioning. Jurnal Produksi Tanaman, 3(6), 518–527.
Zanzibar, M. (2007). Pengaruh perlakuan pengusangan dengan uap etanol terhadap penurunan kualitas fisiologi benih akor, merbau dan mindi. Jurnal Penelitian Hutan Tanaman, 4(2), 99–106. https://doi.org/10.20886/jpht.2007.4.2.99-106